[HTML][HTML] Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop
K Ohashi, K Nagata, M Maekawa, T Ishizaki… - Journal of Biological …, 2000 - ASBMB
LIM-kinase 1 (LIMK1) phosphorylates cofilin, an actin-depolymerizing factor, and regulates
actin cytoskeletal reorganization. LIMK1 is activated by the small GTPase Rho and its
downstream protein kinase ROCK. We now report the site of phosphorylation of LIMK1 by
ROCK. In vitro kinase reaction revealed that the active forms of ROCK phosphorylated
LIMK1 on the threonine residue and markedly increased its cofilin-phosphorylating activity.
A LIMK1 mutant (T508A) with replacement of Thr-508 within the activation loop of the kinase …
actin cytoskeletal reorganization. LIMK1 is activated by the small GTPase Rho and its
downstream protein kinase ROCK. We now report the site of phosphorylation of LIMK1 by
ROCK. In vitro kinase reaction revealed that the active forms of ROCK phosphorylated
LIMK1 on the threonine residue and markedly increased its cofilin-phosphorylating activity.
A LIMK1 mutant (T508A) with replacement of Thr-508 within the activation loop of the kinase …
