[HTML][HTML] Subunit Composition of Brain Voltage-gated Potassium Channels Determined by Hongotoxin-1, a Novel Peptide Derived fromCentruroides limbatus Venom
A Koschak, RM Bugianesi, J Mitterdorfer… - Journal of Biological …, 1998 - ASBMB
Five novel peptidyl inhibitors of Shaker-type (K v 1) K+ channels have been purified to
homogeneity from venom of the scorpionCentruroides limbatus. The complete primary
amino acid sequence of the major component, hongotoxin-1 (HgTX 1), has been determined
and confirmed after expression of the peptide inEscherichia coli. HgTX 1 inhibits 125 I-
margatoxin binding to rat brain membranes as well as depolarization-induced 86 Rb+ flux
through homotetrameric K v 1.1, K v 1.2, and K v 1.3 channels stably transfected in HEK-293 …
homogeneity from venom of the scorpionCentruroides limbatus. The complete primary
amino acid sequence of the major component, hongotoxin-1 (HgTX 1), has been determined
and confirmed after expression of the peptide inEscherichia coli. HgTX 1 inhibits 125 I-
margatoxin binding to rat brain membranes as well as depolarization-induced 86 Rb+ flux
through homotetrameric K v 1.1, K v 1.2, and K v 1.3 channels stably transfected in HEK-293 …
