[HTML][HTML] Regulation of the dephosphorylation of Stat6: participation of Tyr-713 in the interleukin-4 receptor α, the tyrosine phosphatase SHP-1, and the proteasome

EM Hanson, H Dickensheets, CK Qu… - Journal of Biological …, 2003 - ASBMB
Signal transducer and activator of transcription 6 (Stat6) plays an important role in
interleukin (IL)-4-induced responses. To analyze the regulation of Stat6 phosphorylation,
cells were cultured in the continuous presence of IL-4 or after a pulse and washout. In the
continual presence of IL-4, Stat6 remained phosphorylated for an extended period. After IL-4
removal and inhibition of the Janus family kinase, tyrosine-phosphorylated Stat6 decayed at
a rate dependent upon the length of IL-4 stimulation. The decay of tyrosine-phosphorylated …