Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction
Biomaterials, 2015•Elsevier
Cardiac cell therapy suffers from limitations related to poor engraftment and significant cell
death after transplantation. In this regard, ex vivo tissue engineering is a tool that has been
demonstrated to increase cell retention and survival. The aim of our study was to evaluate
the therapeutic potential of a 3D-printed patch composed of human cardiac-derived
progenitor cells (hCMPCs) in a hyaluronic acid/gelatin (HA/gel) based matrix. hCMPCs were
printed in the HA/gel matrix (30× 10 6 cells/ml) to form a biocomplex made of six …
death after transplantation. In this regard, ex vivo tissue engineering is a tool that has been
demonstrated to increase cell retention and survival. The aim of our study was to evaluate
the therapeutic potential of a 3D-printed patch composed of human cardiac-derived
progenitor cells (hCMPCs) in a hyaluronic acid/gelatin (HA/gel) based matrix. hCMPCs were
printed in the HA/gel matrix (30× 10 6 cells/ml) to form a biocomplex made of six …
Abstract
Cardiac cell therapy suffers from limitations related to poor engraftment and significant cell death after transplantation. In this regard, ex vivo tissue engineering is a tool that has been demonstrated to increase cell retention and survival. The aim of our study was to evaluate the therapeutic potential of a 3D-printed patch composed of human cardiac-derived progenitor cells (hCMPCs) in a hyaluronic acid/gelatin (HA/gel) based matrix. hCMPCs were printed in the HA/gel matrix (30 × 106 cells/ml) to form a biocomplex made of six perpendicularly printed layers with a surface of 2 × 2 cm and thickness of 400 μm, in which they retained their viability, proliferation and differentiation capability. The printed biocomplex was transplanted in a mouse model of myocardial infarction (MI). The application of the patch led to a significant reduction in adverse remodeling and preservation of cardiac performance as was shown by both MRI and histology. Furthermore, the matrix supported the long-term in vivo survival and engraftment of hCMPCs, which exhibited a temporal increase in cardiac and vascular differentiation markers over the course of the 4 week follow-up period. Overall, we developed an effective and translational approach to enhance hCMPC delivery and action in the heart.
Elsevier