The acute effect of clamped hyperglycemia on the urinary excretion of inflammatory cytokines/chemokines in uncomplicated type 1 diabetes: a pilot study
DZI Cherney, JW Scholey, E Sochett, TJ Bradley… - Diabetes …, 2011 - Am Diabetes Assoc
Diabetes care, 2011•Am Diabetes Assoc
OBJECTIVE Acute glycemic variability contributes to diabetic complications potentially
through induction of inflammation. Our objective was to determine whether acute
hyperglycemia affects urinary secretion of inflammatory cytokines/chemokines in humans
with uncomplicated type 1 diabetes. RESEARCH DESIGN AND METHODS Blood pressure,
renal hemodynamics (inulin and paraaminohippurate clearances), and urine samples were
obtained after 6 h of clamped euglycemia (4–6 mmol/l) and hyperglycemia (9–11 mmol/l) on …
through induction of inflammation. Our objective was to determine whether acute
hyperglycemia affects urinary secretion of inflammatory cytokines/chemokines in humans
with uncomplicated type 1 diabetes. RESEARCH DESIGN AND METHODS Blood pressure,
renal hemodynamics (inulin and paraaminohippurate clearances), and urine samples were
obtained after 6 h of clamped euglycemia (4–6 mmol/l) and hyperglycemia (9–11 mmol/l) on …
OBJECTIVE
Acute glycemic variability contributes to diabetic complications potentially through induction of inflammation. Our objective was to determine whether acute hyperglycemia affects urinary secretion of inflammatory cytokines/chemokines in humans with uncomplicated type 1 diabetes.
RESEARCH DESIGN AND METHODS
Blood pressure, renal hemodynamics (inulin and paraaminohippurate clearances), and urine samples were obtained after 6 h of clamped euglycemia (4–6 mmol/l) and hyperglycemia (9–11 mmol/l) on two consecutive days in subjects with type 1 diabetes (n = 25). Forty-two urinary cytokines/chemokines were measured using a Luminex platform.
RESULTS
Clamped hyperglycemia produced an expected increase in glomerular filtration rate (131 ± 4 to 148 ± 8 ml/min/1.73 m2). Clamped hyperglycemia was associated with significant increases in urinary eotaxin, fibroblast growth factor-2, granulocyte-macrophage colony-stimulating factor, interferon-α 2, interleukin-2 and -12, monocyte chemoattractant protein-3, macrophage-derived chemokine, macrophage inflammatory protein-1α, platelet-derived growth factor, tumor necrosis factor-α, and CD40 ligand (P < 0.05).
CONCLUSIONS
Acute hyperglycemia results in increased urinary excretion of inflammatory cytokines/chemokines in humans with uncomplicated type 1 diabetes, and this may contribute to kidney injury.
Am Diabetes Assoc