Expression of novel podocyte-associated proteins sult1b1 and ankrd25

X Xu, J Patrakka, L Sistani, M Uhlen… - Nephron Experimental …, 2011 - karger.com
X Xu, J Patrakka, L Sistani, M Uhlen, H Jalanko, C Betsholtz, K Tryggvason
Nephron Experimental Nephrology, 2011karger.com
Background/Aims: Podocytes have a unique function in the renal ultrafiltration that is
achieved by expressing proteins that are highly specific to podocytes. In this study, we
identified two novel podocyte-associated proteins. Methods: The expression of sult1b1 and
ankrd25 in mouse tissues was studied by RT-PCR. The protein expression was studied by
generating polyclonal antibodies that were used in Western blotting and
immunohistochemistry. Results: By RT-PCR we detected sult1b1 expression only in …
Background/Aims
Podocytes have a unique function in the renal ultrafiltration that is achieved by expressing proteins that are highly specific to podocytes. In this study, we identified two novel podocyte-associated proteins.
Methods
The expression of sult1b1 and ankrd25 in mouse tissues was studied by RT-PCR. The protein expression was studied by generating polyclonal antibodies that were used in Western blotting and immunohistochemistry.
Results
By RT-PCR we detected sult1b1 expression only in glomerular, liver and brain tissues. By immunohistochemistry, sult1b1 was detected in the kidney exclusively in the Golgi apparatus of the podocyte. No expression outside the glomerulus was observed in the kidney. The ankrd25 transcript was detected in most mouse tissues analyzed by RT-PCR. In the kidney, however, immunohistochemistry showed that this protein was expressed only by podocyte, mesangial, and smooth muscle cells. In podocytes, ankrd25 was localized to foot processes.
Conclusions
Identification of these two novel glomerulus-associated proteins opens up possibilities to investigate their role in the renal filter physiology and diseases. We speculate that sult1b1 may be involved in the sulfonylation of podocyte protein podocalyxin, whereas ankrd25 may contribute to controlling actin dynamics in podocyte foot processes.
Karger