[HTML][HTML] Evidence of the indirect formation of the catecholic intermediate substrate responsible for the autoactivation kinetics of tyrosinase
CJ Cooksey, PJ Garratt, EJ Land, S Pavel… - Journal of Biological …, 1997 - ASBMB
Tyrosinase (EC 1.14. 18.1) exhibits unusual kinetic properties in the oxidation of monohydric
phenol substrates consisting of a lag period that increases with increasing substrate
concentration. The cause of this is an autocatalytic process dependent on the generation of
a dihydric phenol substrate, which acts as an activator of the enzyme. Experiments with N-
substituted dihydric phenol substrates (N-methyldopamine, N-acetyldopamine) demonstrate
that oxygen consumption is retarded in the N-acetyl substituted material due to a diminished …
phenol substrates consisting of a lag period that increases with increasing substrate
concentration. The cause of this is an autocatalytic process dependent on the generation of
a dihydric phenol substrate, which acts as an activator of the enzyme. Experiments with N-
substituted dihydric phenol substrates (N-methyldopamine, N-acetyldopamine) demonstrate
that oxygen consumption is retarded in the N-acetyl substituted material due to a diminished …