Activated, but not resting, T cells can be recognized and killed by syngeneic NK cells
BA Rabinovich, J Li, J Shannon, R Hurren… - The Journal of …, 2003 - journals.aai.org
BA Rabinovich, J Li, J Shannon, R Hurren, J Chalupny, D Cosman, RG Miller
The Journal of Immunology, 2003•journals.aai.orgWe demonstrate that IL-2-activated NK cells or lymphokine-activated killer cells recognize
and kill syngeneic CD4+ and CD8+ T cells that have been activated by APCs. Induction with
APC required TCR-specific Ag, and lysis was perforin mediated. Brefeldin A, which disrupts
protein transport, inhibited the sensitivity induced by activation. In BALB/c, expression of
NKG2D ligands correlated with lysis and could be inhibited by brefeldin A. As well, addition
of anti-NKG2D mAb to a killing assay completely abrogated lysis. Transduction of mouse …
and kill syngeneic CD4+ and CD8+ T cells that have been activated by APCs. Induction with
APC required TCR-specific Ag, and lysis was perforin mediated. Brefeldin A, which disrupts
protein transport, inhibited the sensitivity induced by activation. In BALB/c, expression of
NKG2D ligands correlated with lysis and could be inhibited by brefeldin A. As well, addition
of anti-NKG2D mAb to a killing assay completely abrogated lysis. Transduction of mouse …
Abstract
We demonstrate that IL-2-activated NK cells or lymphokine-activated killer cells recognize and kill syngeneic CD4+ and CD8+ T cells that have been activated by APCs. Induction with APC required TCR-specific Ag, and lysis was perforin mediated. Brefeldin A, which disrupts protein transport, inhibited the sensitivity induced by activation. In BALB/c, expression of NKG2D ligands correlated with lysis and could be inhibited by brefeldin A. As well, addition of anti-NKG2D mAb to a killing assay completely abrogated lysis. Transduction of mouse NKG2D into a human NK cell line, YTSeco, conferred upon it the ability to kill activated BALB/c T cells, indicating that NKG2D is necessary for recognition. Our data provide a basis for studying a role for NK cells in T cell regulation.
journals.aai.org