A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy
S Oehen, K Brduscha-Riem, A Oxenius… - Journal of immunological …, 1997 - Elsevier
In this report we describe a simple method using the vital dye 5-(and 6-) carboxyfluorescein
diacetate succinimidyl ester (CFSE) to follow splenic graft rejection by flow cytometry. CFSE-
labelled spleen cell suspensions were injected intravenously into various recipients and
blood samples were taken at different time points to follow the transferred cells. We found
that the labelled cells could be readily detected by flow cytometry for up to eleven weeks.
The loss of these labelled cells in various transfusion experiments with different major and …
diacetate succinimidyl ester (CFSE) to follow splenic graft rejection by flow cytometry. CFSE-
labelled spleen cell suspensions were injected intravenously into various recipients and
blood samples were taken at different time points to follow the transferred cells. We found
that the labelled cells could be readily detected by flow cytometry for up to eleven weeks.
The loss of these labelled cells in various transfusion experiments with different major and …