Mapping the type I collagen-binding site on pigment epithelium-derived factor: Implications for its antiangiogenic activity
C Meyer, L Notari, SP Becerra - Journal of Biological Chemistry, 2002 - ASBMB
Pigment epithelium-derived factor (PEDF), a neurotrophic and antiangiogenic serpin, is
identified in tissues rich in collagen, eg cornea, vitreous, bone, and cartilage. We show that
recombinant human PEDF formed complexes with collagens from the bovine cornea and
vitreous. We have examined the direct binding of PEDF to collagen I and found that
interactions were ionic in nature and occurred when PEDF and collagen I were both in
solution, when either one was immobilized, or even when collagen I was denatured under …
identified in tissues rich in collagen, eg cornea, vitreous, bone, and cartilage. We show that
recombinant human PEDF formed complexes with collagens from the bovine cornea and
vitreous. We have examined the direct binding of PEDF to collagen I and found that
interactions were ionic in nature and occurred when PEDF and collagen I were both in
solution, when either one was immobilized, or even when collagen I was denatured under …
