MICROCHIMERIC CELLS FROM THE PERIPHERAL BLOOD ASSOCIATED WITH CARDIAC GRAFTS ARE BONE MARROW DERIVED, LONG-LIVED AND …
Y Yano, M Hara, T Miyahara, K Shibata… - …, 2001 - journals.lww.com
Y Yano, M Hara, T Miyahara, K Shibata, T Onitsuka, Y Nawa, XK Li, S Suzuki, H Amemiya…
Transplantation, 2001•journals.lww.comBackground. Although it has been well established that themicrochimerism occurs in the
peripheral blood of the recipients after varioussettings in both clinical and experimental
organ transplantation, nevertheless, their roles in inducing and maintaining acquired
transplantationtolerance are controversial. Furthermore, regarding the cell lineages, kinetics,
and functions of the cells that constitute the microchimerism afterorgan transplantation, solid
information is notavailable. Methods. Using rat heterotopic heart isografts from bone …
peripheral blood of the recipients after varioussettings in both clinical and experimental
organ transplantation, nevertheless, their roles in inducing and maintaining acquired
transplantationtolerance are controversial. Furthermore, regarding the cell lineages, kinetics,
and functions of the cells that constitute the microchimerism afterorgan transplantation, solid
information is notavailable. Methods. Using rat heterotopic heart isografts from bone …
Abstract
Background.
Although it has been well established that themicrochimerism occurs in the peripheral blood of the recipients after varioussettings in both clinical and experimental organ transplantation, nevertheless, their roles in inducing and maintaining acquired transplantationtolerance are controversial. Furthermore, regarding the cell lineages, kinetics, and functions of the cells that constitute the microchimerism afterorgan transplantation, solid information is notavailable.
Methods.
Using rat heterotopic heart isografts from bone marrowchimeras between cross-sex and applying polymerase chain reaction withspecific primers to rat sex determining region of Y chromosome, a relationshipbetween a state of microchimerism and induction as well as maintenance ofacquired tolerance to HY antigen wereexamined.
Results.
Microchimeric cells of the peripheral blood (MCPB) aftercardiac grafting contain bone marrow-derived and radiation-sensitive cells. Furthermore, removal of the primary cardiac grafts revealed that microchimericcells in the peripheral blood are long-lived cells, ie, more than 6 months. When the female rats that had contained long-lasting MCPB, were innoculatedwith syngeneic male dendritic cells, failure to sensitize female toward malespecific antigen HY was found tooccur.
Conclusions.
Thus it was suggested that radiation-sensitive, bone marrowderived, long-lived MCPB play a significant role in maintaining acquiredtransplantation tolerance to minor histocompatibility antigenH-Y.
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