Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation
M Kuwana, Y Okazaki, H Kodama… - Journal of Leucocyte …, 2003 - academic.oup.com
M Kuwana, Y Okazaki, H Kodama, K Izumi, H Yasuoka, Y Ogawa, Y Kawakami, Y Ikeda
Journal of Leucocyte Biology, 2003•academic.oup.comCirculating CD14+ monocytes are precursors of phagocytes, such as macrophages and
dendritic cells. Here we report primitive cells with a fibroblast-like morphology derived from
human peripheral blood CD14+ monocytes that can differentiate into several distinct
mesenchymal cell lineages. We named this cell population monocyte-derived mesenchymal
progenitor (MOMP). MOMPs were obtained in vitro from human peripheral blood
mononuclear cells cultured on fibronectin in the presence of fetal bovine serum alone as a …
dendritic cells. Here we report primitive cells with a fibroblast-like morphology derived from
human peripheral blood CD14+ monocytes that can differentiate into several distinct
mesenchymal cell lineages. We named this cell population monocyte-derived mesenchymal
progenitor (MOMP). MOMPs were obtained in vitro from human peripheral blood
mononuclear cells cultured on fibronectin in the presence of fetal bovine serum alone as a …
Abstract
Circulating CD14+ monocytes are precursors of phagocytes, such as macrophages and dendritic cells. Here we report primitive cells with a fibroblast-like morphology derived from human peripheral blood CD14+ monocytes that can differentiate into several distinct mesenchymal cell lineages. We named this cell population monocyte-derived mesenchymal progenitor (MOMP). MOMPs were obtained in vitro from human peripheral blood mononuclear cells cultured on fibronectin in the presence of fetal bovine serum alone as a source of growth factors. MOMPs had a unique molecular phenotype–CD14+CD45+CD34+ type I collagen+–and showed mixed morphologic and molecular features of monocytes and endothelial and mesenchymal cells. MOMPs were found to be derived from a subset of circulating CD14+ monocytes, and their differentiation required that they bind fibronectin and be exposed to one or more soluble factors derived from peripheral blood CD14− cells. MOMPs could be expanded in culture without losing their original phenotype for up to five passages. The induction of MOMPs to differentiate along multiple limb-bud mesodermal lineages resulted in the expression of genes and proteins specific for osteoblasts, skeletal myoblasts, chondrocytes, and adipocytes. Our findings represent the first evidence that human circulating CD14+ monocytes are a source of progenitors that exhibit mesenchymal cell differentiation.
Oxford University Press