Excessive activation of matrix metalloproteinases coincides with left ventricular remodeling during transition from hypertrophy to heart failure in hypertensive rats
Y Iwanaga, T Aoyama, Y Kihara, Y Onozawa… - Journal of the American …, 2002 - jacc.org
Y Iwanaga, T Aoyama, Y Kihara, Y Onozawa, T Yoneda, S Sasayama
Journal of the American College of Cardiology, 2002•jacc.orgObjectives: We sought to elucidate how the local activation of matrix metalloproteinases
(MMPs) is balanced by that of the endogenous tissue inhibitors of MMP (TIMPs) during left
ventricular (LV) remodeling. Background: Although it is known that the extracellular matrix
(ECM) must be altered during LV remodeling, its local regulation has not been fully
elucidated. Methods: In Dahl salt-sensitive rats with hypertension, in which a stage of
concentric, compensated left ventricular hypertrophy (LVH) at 11 weeks is followed by a …
(MMPs) is balanced by that of the endogenous tissue inhibitors of MMP (TIMPs) during left
ventricular (LV) remodeling. Background: Although it is known that the extracellular matrix
(ECM) must be altered during LV remodeling, its local regulation has not been fully
elucidated. Methods: In Dahl salt-sensitive rats with hypertension, in which a stage of
concentric, compensated left ventricular hypertrophy (LVH) at 11 weeks is followed by a …
Objectives
We sought to elucidate how the local activation of matrix metalloproteinases (MMPs) is balanced by that of the endogenous tissue inhibitors of MMP (TIMPs) during left ventricular (LV) remodeling.
Background
Although it is known that the extracellular matrix (ECM) must be altered during LV remodeling, its local regulation has not been fully elucidated.
Methods
In Dahl salt-sensitive rats with hypertension, in which a stage of concentric, compensated left ventricular hypertrophy (LVH) at 11 weeks is followed by a distinct stage of congestive heart failure (CHF) with LV enlargement and dysfunction at 17 weeks, we determined protein and messenger ribonucleic acid (mRNA) levels of LV myocardial TIMP-2 and -4 and MMP-2, as well as their concomitant activities.
Results
No changes were found at the LVH stage. However, during the transition to CHF, TIMP-2 and -4 activities, protein and mRNA levels were all sharply increased. At the same time, the MMP-2 mRNA and protein levels and activities, as determined by gelatin zymography, as well as by an antibody capture assay, showed a substantial increase during the transition to CHF. The net MMP activities were closely related to increases in LV diameter (r = 0.763) and to systolic wall stress (r = 0.858) in vivo.
Conclusions
Both TIMPs and MMP-2 remained inactive during hypertrophy, per se; they were activated during the transition to CHF. At this time, the activation of MMP-2 surpassed that of TIMPs, possibly resulting in ECM breakdown and progression of LV enlargement.
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