[HTML][HTML] Transmembrane topology of human glucose 6-phosphate transporter
CJ Pan, B Lin, JY Chou - Journal of Biological Chemistry, 1999 - ASBMB
Glycogen storage disease type 1b is caused by a deficiency in a glucose 6-phosphate
transporter (G6PT) that translocates glucose 6-phosphate from the cytoplasm to the
endoplasmic reticulum lumen where the active site of glucose 6-phosphatase is situated.
Using amino-and carboxyl-terminal tagged G6PT, we demonstrate that proteolytic digestion
of intact microsomes resulted in the cleavage of both tags, indicating that both termini of
G6PT face the cytoplasm. This is consistent with ten and twelve transmembrane domain …
transporter (G6PT) that translocates glucose 6-phosphate from the cytoplasm to the
endoplasmic reticulum lumen where the active site of glucose 6-phosphatase is situated.
Using amino-and carboxyl-terminal tagged G6PT, we demonstrate that proteolytic digestion
of intact microsomes resulted in the cleavage of both tags, indicating that both termini of
G6PT face the cytoplasm. This is consistent with ten and twelve transmembrane domain …
