N-linked glycosylation of the HIV type-1 gp120 envelope glycoprotein as a major determinant of CCR5 and CXCR4 coreceptor utilization
G Pollakis, S Kang, A Kliphuis, MIM Chalaby… - Journal of Biological …, 2001 - ASBMB
The variable V1V2 and V3 regions of the human immunodeficiency virus type-1 (HIV-1)
envelope glycoprotein (gp120) can influence viral coreceptor usage. To substantiate this we
generated isogenic HIV-1 molecularly cloned viruses that were composed of the HxB2
envelope backbone containing the V1V2 and V3 regions from viruses isolated from a patient
progressing to disease. We show that the V3 amino acid charge per se had little influence
on altering the virus coreceptor phenotype. The V1V2 region and itsN-linked glycosylation …
envelope glycoprotein (gp120) can influence viral coreceptor usage. To substantiate this we
generated isogenic HIV-1 molecularly cloned viruses that were composed of the HxB2
envelope backbone containing the V1V2 and V3 regions from viruses isolated from a patient
progressing to disease. We show that the V3 amino acid charge per se had little influence
on altering the virus coreceptor phenotype. The V1V2 region and itsN-linked glycosylation …