[HTML][HTML] Src-catalyzed phosphorylation of c-Cbl leads to the interdependent ubiquitination of both proteins
The protooncogene c-Cbl has recently emerged as an E3 ubiquitin ligase for activated
receptor tyrosine kinases. We report here that c-Cbl also mediates the ubiquitination of
another protooncogene, the non-receptor tyrosine kinase c-Src, as well as of itself. The c-Cbl-
dependent ubiquitination of Src and c-Cbl requires c-Cbl's RING finger, Src kinase activity,
and c-Cbl's tyrosine phosphorylation, probably on Tyr-371. In vitro, c-Cbl forms a stable
complex with the ubiquitin-conjugating enzyme UbcH7, but active Src destabilizes this …
receptor tyrosine kinases. We report here that c-Cbl also mediates the ubiquitination of
another protooncogene, the non-receptor tyrosine kinase c-Src, as well as of itself. The c-Cbl-
dependent ubiquitination of Src and c-Cbl requires c-Cbl's RING finger, Src kinase activity,
and c-Cbl's tyrosine phosphorylation, probably on Tyr-371. In vitro, c-Cbl forms a stable
complex with the ubiquitin-conjugating enzyme UbcH7, but active Src destabilizes this …