Quantitation of human immunodeficiency virus type 1 RNA in cell free seminal plasma: comparison of NASBA™ with Amplicor™ reverse transcription-PCR …
JR Dyer, BL Gilliam, JJ Eron Jr, L Grosso… - Journal of virological …, 1996 - Elsevier
Human immunodeficiency virus type 1 (HIV-1) is transmitted by infected males in semen.
However, the inoculum required for infection is unknown. The ability to collect such
information will rely on the availability of reliable quantitative assays of HIV-1 in semen. We
examined the comparative performance of NASBA™ and Amplicor Monitor™ RT-PCR in
quantifying HIV-1 RNA in cell free seminal plasma from seropositive men and correlated the
results obtained with viral titres measured by a seminal cell quantitative microculture (QMC) …
However, the inoculum required for infection is unknown. The ability to collect such
information will rely on the availability of reliable quantitative assays of HIV-1 in semen. We
examined the comparative performance of NASBA™ and Amplicor Monitor™ RT-PCR in
quantifying HIV-1 RNA in cell free seminal plasma from seropositive men and correlated the
results obtained with viral titres measured by a seminal cell quantitative microculture (QMC) …