Three-dimensional supramolecular organization of the extracellular matrix in human and rabbit corneal stroma, as revealed by ultrarapid-freezing and deep-etching …
M Hirsch, G Prenant, G Renard - Experimental eye research, 2001 - Elsevier
The present work was carried out to clarify the three-dimensional fine structure of
extracellular matrix in the cornea, using ultrarapid-freezing and deep-etching methods for
electron microscopy. Fresh and glutaraldehyde-fixed samples of human and rabbit posterior
corneas were ultrarapidly-frozen onto a copper block cooled by liquid helium or liquid
nitrogen, freeze-fractured, deeply etched for 8–10min and rotary replicated with platinum-
carbon. Replicas were examined in a transmission electron microscope equipped with a …
extracellular matrix in the cornea, using ultrarapid-freezing and deep-etching methods for
electron microscopy. Fresh and glutaraldehyde-fixed samples of human and rabbit posterior
corneas were ultrarapidly-frozen onto a copper block cooled by liquid helium or liquid
nitrogen, freeze-fractured, deeply etched for 8–10min and rotary replicated with platinum-
carbon. Replicas were examined in a transmission electron microscope equipped with a …
