[HTML][HTML] Targeted correction of an episomal gene in mammalian cells by a short DNA fragment tethered to a triplex-forming oligonucleotide
PP Chan, M Lin, AF Faruqi, J Powell… - Journal of Biological …, 1999 - ASBMB
Triplex-forming oligonucleotides (TFOs) can bind to polypurine/polypyrimidine regions in
DNA in a sequence-specific manner and provoke DNA repair. We have coupled a TFO to a
short donor fragment of DNA that shares homology to a selected gene as a strategy to
mediate gene targeting and correction. In this bifunctional oligonucleotide, the TFO domain
is designed to bind the target gene and stimulate repair and recombination, with the donor
domain positioned for recombination and information transfer. A series of these tethered …
DNA in a sequence-specific manner and provoke DNA repair. We have coupled a TFO to a
short donor fragment of DNA that shares homology to a selected gene as a strategy to
mediate gene targeting and correction. In this bifunctional oligonucleotide, the TFO domain
is designed to bind the target gene and stimulate repair and recombination, with the donor
domain positioned for recombination and information transfer. A series of these tethered …