Repairing the Sickle Cell Mutation: I. SPECIFIC COVALENT BINDING OF A PHOTOREACTIVE THIRD STRAND TO THE MUTATED BASE PAIR
S Broitman, O Amosova, NG Dolinnaya… - Journal of Biological …, 1999 - ASBMB
A DNA third strand with a 3′-psoralen substituent was designed to form a triplex with the
sequence downstream of the T· A mutant base pair of the human sickle cell β-globin gene.
Triplex-mediated psoralen modification of the mutant T residue was sought as an approach
to gene repair. The 24-nucleotide purine-rich target sequence switches from one strand to
the other and has four pyrimidine interruptions. Therefore, a third strand sequence favorable
to two triplex motifs was used, one parallel and the other antiparallel to it. To cope with the …
sequence downstream of the T· A mutant base pair of the human sickle cell β-globin gene.
Triplex-mediated psoralen modification of the mutant T residue was sought as an approach
to gene repair. The 24-nucleotide purine-rich target sequence switches from one strand to
the other and has four pyrimidine interruptions. Therefore, a third strand sequence favorable
to two triplex motifs was used, one parallel and the other antiparallel to it. To cope with the …
