Targeted gene knockout by 2′-O-aminoethyl modified triplex forming oligonucleotides
N Puri, A Majumdar, B Cuenoud, F Natt, P Martin… - Journal of Biological …, 2001 - ASBMB
Triplex forming oligonucleotides (TFOs) are of interest because of their potential for facile
gene targeting. However, the failure of TFOs to bind target sequences at physiological pH
and Mg 2+ concentration has limited their biological applications. Recently, pyrimidine TFOs
with 2′-O-aminoethyl (AE) substitutions were shown to have enhanced kinetics and
stability of triplex formation (Cuenoud, B., Casset, F., Husken, D., Natt, F., Wolf, RM, Altmann,
KH, Martin, P., and Moser HE (1998) Angew. Chem. Int. Ed. 37, 1288–1291). We have …
gene targeting. However, the failure of TFOs to bind target sequences at physiological pH
and Mg 2+ concentration has limited their biological applications. Recently, pyrimidine TFOs
with 2′-O-aminoethyl (AE) substitutions were shown to have enhanced kinetics and
stability of triplex formation (Cuenoud, B., Casset, F., Husken, D., Natt, F., Wolf, RM, Altmann,
KH, Martin, P., and Moser HE (1998) Angew. Chem. Int. Ed. 37, 1288–1291). We have …
