Historical Perspective-s-The origin of DIA dates back 60 years. Caspersson'is credited with
having performed the first cytophotometric measurements in 1935. In 1924, Feulgen,?
whose name is synonymous with the stain used for DNA ploidy analysis by DIA, published
his work on a dye that stoichiometrically binds to hydrolyzed nucleic acids. During the past
20 years, automated morphometry evolved to its current level of sophistication because of
insights derived from molecular biology and improved image capturing and computer …