[HTML][HTML] The ubiquitin-proteasome pathway mediates the regulated degradation of mammalian 3-hydroxy-3-methylglutaryl-coenzyme A reductase
T Ravid, R Doolman, R Avner, D Harats… - Journal of Biological …, 2000 - ASBMB
3-Hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), the key regulatory enzyme in
the mevalonate (MVA) pathway, is rapidly degraded in mammalian cells supplemented with
sterols or MVA. This accelerated turnover was blocked byN-acetyl-leucyl-leucyl-norleucinal
(ALLN), MG-132, and lactacystin, and to a lesser extent byN-acetyl-leucyl-leucyl-methional
(ALLM), indicating the involvement of the 26 S proteasome. Proteasome inhibition led to
enhanced accumulation of high molecular weight polyubiquitin conjugates of HMGR and of …
the mevalonate (MVA) pathway, is rapidly degraded in mammalian cells supplemented with
sterols or MVA. This accelerated turnover was blocked byN-acetyl-leucyl-leucyl-norleucinal
(ALLN), MG-132, and lactacystin, and to a lesser extent byN-acetyl-leucyl-leucyl-methional
(ALLM), indicating the involvement of the 26 S proteasome. Proteasome inhibition led to
enhanced accumulation of high molecular weight polyubiquitin conjugates of HMGR and of …
