[HTML][HTML] Ligand-, cell-, and estrogen receptor subtype (α/β)-dependent activation at GC-rich (Sp1) promoter elements
B Saville, M Wormke, F Wang, T Nguyen… - Journal of Biological …, 2000 - ASBMB
17β-Estradiol (E2) induces expression of several genes via estrogen receptor (ER)-Sp1
protein interactions with GC-rich promoter elements in which Sp1 but not ER binds DNA.
This study reports the ligand-and cell context-dependent ER α/Sp1 and ER β/Sp1 action
using an E2-responsive construct (pSp1) containing a GC-rich promoter. Both ER α and ER
β proteins physically interact with Sp1 (coimmunoprecipitation) and preferentially bind to the
C-terminal region of this protein in pull-down assays. E2-and antiestrogen-dependent …
protein interactions with GC-rich promoter elements in which Sp1 but not ER binds DNA.
This study reports the ligand-and cell context-dependent ER α/Sp1 and ER β/Sp1 action
using an E2-responsive construct (pSp1) containing a GC-rich promoter. Both ER α and ER
β proteins physically interact with Sp1 (coimmunoprecipitation) and preferentially bind to the
C-terminal region of this protein in pull-down assays. E2-and antiestrogen-dependent …
