Frequency-encoding Thr17 phospholamban phosphorylation is independent of Ser16 phosphorylation in cardiac myocytes
D Hagemann, M Kuschel, T Kuramochi, W Zhu… - Journal of Biological …, 2000 - ASBMB
Both Ser 16 and Thr 17 of phospholamban (PLB) are phosphorylated, respectively, by cAMP-
dependent protein kinase (PKA) and Ca 2+/calmodulin-dependent protein kinase II
(CaMKII). PLB phosphorylation relieves cardiac sarcoplasmic reticulum Ca 2+ pump from
inhibition by PLB. Previous studies have suggested that phosphorylation of Ser 16 by PKA is
a prerequisite for Thr 17 phosphorylation by CaMKII and is essential to the relaxant effect of
β-adrenergic stimulation. To determine the role of Thr 17 PLB phosphorylation, we …
dependent protein kinase (PKA) and Ca 2+/calmodulin-dependent protein kinase II
(CaMKII). PLB phosphorylation relieves cardiac sarcoplasmic reticulum Ca 2+ pump from
inhibition by PLB. Previous studies have suggested that phosphorylation of Ser 16 by PKA is
a prerequisite for Thr 17 phosphorylation by CaMKII and is essential to the relaxant effect of
β-adrenergic stimulation. To determine the role of Thr 17 PLB phosphorylation, we …
