The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes
CG Larsen, AO Anderson, E Appella, JJ Oppenheim… - Science, 1989 - science.org
CG Larsen, AO Anderson, E Appella, JJ Oppenheim, K Matsushima
Science, 1989•science.orgT lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned
media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a
sequence of chromatography procedures. The amino-terminal amino acid sequence of the
purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and
recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro
supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas …
media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a
sequence of chromatography procedures. The amino-terminal amino acid sequence of the
purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and
recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro
supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas …
T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.
AAAS