[HTML][HTML] The PEST domain of IκBα is necessary and sufficient forin vitro degradation by μ-calpain
SD Shumway, M Maki, S Miyamoto - Journal of Biological Chemistry, 1999 - ASBMB
Polypeptide sequences enriched in proline (P), glutamate (E), serine (S), and threonine (T),
dubbed PEST domains, are proposed to expedite the degradation of proteins. The
proteolysis of one PEST-containing protein, IκBα, is prerequisite to the activation of the
transcription factor NF-κB. Two mechanisms of IκBα degradation in vivo have been
described, one well characterized through the ubiquitin-proteasome pathway, and another
less characterized through calpain. In this report, a mutational analysis was done to identify …
dubbed PEST domains, are proposed to expedite the degradation of proteins. The
proteolysis of one PEST-containing protein, IκBα, is prerequisite to the activation of the
transcription factor NF-κB. Two mechanisms of IκBα degradation in vivo have been
described, one well characterized through the ubiquitin-proteasome pathway, and another
less characterized through calpain. In this report, a mutational analysis was done to identify …
