Distinct roles for cyclin-dependent kinases in cell cycle control
S Van den Heuvel, E Harlow - Science, 1993 - science.org
S Van den Heuvel, E Harlow
Science, 1993•science.orgThe key cell-cycle regulator Cdc2 belongs to a family of cyclin-dependent kinases in higher
eukaryotes. Dominant-negative mutations were used to address the requirement for kinases
of this family in progression through the human cell cycle. A dominant-negative Cdc2 mutant
arrested cells at the G2 to M phase transition, whereas mutants of the cyclin-dependent
kinases Cdk2 and Cdk3 caused a G1 block. The mutant phenotypes were specifically
rescued by the corresponding wild-type kinases. These data reveal that Cdk3, in addition to …
eukaryotes. Dominant-negative mutations were used to address the requirement for kinases
of this family in progression through the human cell cycle. A dominant-negative Cdc2 mutant
arrested cells at the G2 to M phase transition, whereas mutants of the cyclin-dependent
kinases Cdk2 and Cdk3 caused a G1 block. The mutant phenotypes were specifically
rescued by the corresponding wild-type kinases. These data reveal that Cdk3, in addition to …
The key cell-cycle regulator Cdc2 belongs to a family of cyclin-dependent kinases in higher eukaryotes. Dominant-negative mutations were used to address the requirement for kinases of this family in progression through the human cell cycle. A dominant-negative Cdc2 mutant arrested cells at the G2 to M phase transition, whereas mutants of the cyclin-dependent kinases Cdk2 and Cdk3 caused a G1 block. The mutant phenotypes were specifically rescued by the corresponding wild-type kinases. These data reveal that Cdk3, in addition to Cdc2 and Cdk2, executes a distinct and essential function in the mammalian cell cycle.
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