[HTML][HTML] Induction and Stabilization of IκBα by Nitric Oxide Mediates Inhibition of NF-κB∗
To determine the mechanism (s) by which the endogenous mediator nitric oxide (NO)
inhibits the activation of transcription factor NF-κB, we stimulated human vascular
endothelial cells with tumor necrosis factor-α in the presence of two NO donors, sodium
nitroprusside and S-nitrosoglutathione. Electrophoretic mobility shift assays demonstrated
that both NO donors inhibited NF-κB activation by tumor necrosis factor-α. This effect was not
mediated by guanylyl cyclase activation since the cGMP analogue 8-bromo-cGMP had no …
inhibits the activation of transcription factor NF-κB, we stimulated human vascular
endothelial cells with tumor necrosis factor-α in the presence of two NO donors, sodium
nitroprusside and S-nitrosoglutathione. Electrophoretic mobility shift assays demonstrated
that both NO donors inhibited NF-κB activation by tumor necrosis factor-α. This effect was not
mediated by guanylyl cyclase activation since the cGMP analogue 8-bromo-cGMP had no …